Composite

Part:BBa_K3425140:Design

Designed by: Pedro Nunes   Group: iGEM20_UofUppsala   (2020-10-25)


PydfJ115_GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 749


Design Notes

The RBS used (BBa_B0034) is an E. coli part but it is also known to work in B. subtilis.

When designing composite parts for this chassis, using a double terminator is recommended. Thus, BBa_B0015 was used.

Source

The BBa_E0040 sequence was obtained from the registry and BBa_K3425130 from the chromosome of B. subtilis strain w168.